Journal: PLOS ONE

Article Title: Highly sensitive molecular assay based on Identical Multi-Repeat Sequence (IMRS) algorithm for the detection of Trichomonas vaginalis infection

doi: 10.1371/journal.pone.0317958

Figure Lengend Snippet: Lower limit of detection (LLOD) for the Iso-thermal amplification was calculated using the Probit analysis (A). 2% gel image of amplicons of 100-fold serial dilution of Trichomonas vaginalis DNA template (1, 100bp ladder, 2, 10 2 pg/μl, 3, 1 pg/μl, 4, 10 −2 pg/μl, 5, 10 −4 pg/μl, 6, 10 −6 pg/μl, 7, 10 −8 pg/μl and 8, non-template control). The estimated LLOD for the Isothermal assay was 0.0201 pg/μl.

Article Snippet: Quantitative Genomic DNA from T . vaginalis (ATCC® 30001DQTM) was obtained from the American Type Culture Collection (ATCC) at a concentration of ≥1 x 10 5 copies/μL.

Techniques: Amplification, Serial Dilution, Control

Journal: PLOS ONE

Article Title: Highly sensitive molecular assay based on Identical Multi-Repeat Sequence (IMRS) algorithm for the detection of Trichomonas vaginalis infection

doi: 10.1371/journal.pone.0317958

Figure Lengend Snippet: Lower limit of detection for Trichomonas vaginalis using IMRS primers was 0.03 fg/ μ l (A) and 18S rRNA PCR primers was 0.714pg/ μ L (B) respectively.

Article Snippet: Quantitative Genomic DNA from T . vaginalis (ATCC® 30001DQTM) was obtained from the American Type Culture Collection (ATCC) at a concentration of ≥1 x 10 5 copies/μL.

Techniques:

Journal: Drug Design, Development and Therapy

Article Title: Tanshinone IIA Ameliorates Streptozotocin-Induced Diabetic Nephropathy, Partly by Attenuating PERK Pathway-Induced Fibrosis

doi: 10.2147/DDDT.S257734

Figure Lengend Snippet: Tan IIA down-regulates TGF-β1, TSP-1, Grp78 and CHOP expression in the renal tissues of the diabetic rats. ( A ) Immunohistochemical analysis of TGF-β1, TSP-1, Grp78 and CHOP expression in the renal tissues among five groups and the pooled data from ten sections for each group is summarized. Scale bar: 50 μm (×400). ( B, C ) The expression levels of Grp78 ( B ) and CHOP ( C ) from different treatment groups were determined by qRT-PCR. N = 10; * P <0.05 and ** P <0.01.

Article Snippet: The sections were incubated with primary antibodies against Grp78 (1:100), CHOP (1:50), TGF-β1 (1:100) and TSP-1 (1:100) overnight at 4°C, followed by incubating with biotinylated goat anti-rabbit IgG (1:200, # BA1003; Boster Biological Technology, Wuhan, China) in phosphate-buffered saline (PBS) for 2 h at room temperature.

Techniques: Expressing, Immunohistochemical staining, Quantitative RT-PCR

Journal: Journal of Clinical Investigation

Article Title: Methylation in pericytes after acute injury promotes chronic kidney disease

doi: 10.1172/jci135773

Figure Lengend Snippet: Figure 8. YBX2 expression in pericytes decreased after acute kidney injury. (A) Representative images showing the immunohistochemistry staining of YBX2 in the Ctrl kidneys. Arrows indicate YBX2+ interstitial cells. Scale bars: 25 μm. Original magnification, ×400. (B) Represen- tative images showing Col1a1-GFP+ qPericytes with YBX2 expression in the Ctrl kidney of Col1a1-GFPTg mice. Scale bars: 25 μm. Original magnification, ×400. (C) Representative images showing the immuno histochemistry staining of YBX2 in kidneys on day 7 after IRI-AKI. Arrowheads indicate YBX2+ tubular epithelial cells while asterisks indicate interstitial cells without YBX2. Scale bars: 25 μm. Original magnification, ×400. (D) Dot chart showing the expression of Ybx2 in the Ctrl and day 7 IRI-AKI kidneys. n = 5. (E and F) Dot chart showing the expression of Ybx2 and Acta2 in purified pericytes from the kidneys at the indicated time points after IRI-AKI. Horizontal bars represent the mean, error bars represent the SEM. n = 4. *P < 0.05, **P < 0.01, ***P < 0.001 by 1-way ANOVA with post hoc Tukey’s correction.

Article Snippet: Sections were blocked in 10% normal goat serum for 1 hour, and then incubated with the primary antibodies against YBX2 (13538-1-AP, Proteintech) or nonimmunized rabbit IgG (011- 000-003, Jackson ImmunoResearch Laboratories) at 4°C overnight.

Techniques: Expressing, Immunohistochemistry, Staining, Purification

Journal: Journal of Clinical Investigation

Article Title: Methylation in pericytes after acute injury promotes chronic kidney disease

doi: 10.1172/jci135773

Figure Lengend Snippet: Figure 9. YBX2 repressed the expression of Acta2. (A) Line chart showing the expression of Ybx2 mRNA in the primary kidney pericytes after TGF-β1 exposure and withdrawal at the indicated time points. Data were expressed as the mean ± SEM. n = 3. *P < 0.05 by t test vs. before TGF-β1 withdrawal. (B) Representative images showing the electrophoresis of the PCR products of the Ybx2 gene using MeDIP or input DNA from primary kidney pericytes after TGF-β1 exposure for 24 or 120 hours. Pericytes without TGF-β1 exposure served as the Ctrl. (C) Scheme showing the primer design in the promoter regions of the Acta2 gene. (D) Repre- sentative images showing the electrophoresis of PCR products of the promoter of the Acta2 gene using DNA immunoprecipitated by anti- YBX2 antibody (ChIP) or input DNA from the primary kidney pericytes with or without TGF-β1 exposure for 120 hours. ChIP using isotype IgG served as the Ctrl. (E) Representative Western blot analyses for YBX2, αSMA, and β-actin in pericytes with or without lentiviral transduction for YBX2 expression. (F) Dot chart showing the relative expression of YBX2 and αSMA in pericytes with or without lentiviral transduction for YBX2 expression. Horizontal bars represent the mean, error bars represent the SEM. n = 3. **P < 0.01, ***P < 0.001 by 1-way ANOVA with post hoc Tukey’s correction.

Article Snippet: Sections were blocked in 10% normal goat serum for 1 hour, and then incubated with the primary antibodies against YBX2 (13538-1-AP, Proteintech) or nonimmunized rabbit IgG (011- 000-003, Jackson ImmunoResearch Laboratories) at 4°C overnight.

Techniques: Expressing, Electrophoresis, Methylated DNA Immunoprecipitation, Immunoprecipitation, Western Blot, Transduction